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Can the WIPO Sequence be used without an internet connection?

Yes. WIPO Sequence has been implemented as a stand-alone desktop tool, as opposed to a web application so that applicants can work without being connected to the internet.  However, in order to ensure that you have the latest version, by invoking the auto-update feature work, it is necessary that you are connected to the internet. Additionally the software architecture also provides the potential for the desktop tool implementation to be deployed as a web application in the future.