How do you add DNA primer names using ST.26 qualifiers?
While there is no one right way to include the name of a primer, the possible ways depend on if the sequence to be annotated is amplified by the primer, or if the sequence is the primer itself. Note, I include references below to specific sections of WIPO Standard ST.26.
If the sequence to be annotated is amplified by the primers in question:
- The feature key “primer_bind” (see Annex I, section 5.28) can be used to show where on the sequence the primer(s) bind. A “note” qualifier (see Annex I, section 6.40) can be added to include the name of the primer.
- OR -
- a “PCR_primers” qualifier (see Annex I, section 6.45) can be added to the mandatory “source” feature (see Annex I, section 5.37). The “PCR_primers” qualifier value format includes the name of the primer as well as the sequence of the primer.
If the sequence to be annotated is the primer sequence itself:
- A “note” qualifier could be added to the mandatory “source” feature where the value is the name of the primer.
- OR –
- A “misc_feature” feature key (see Annex I, section 5.14) can be added with a “note” qualifier where the value is the name of the primer.